MMAE ADC EIA Kit DEIABL312 from Creative Diagnostics

Product Specs
ItemMMAE ADC EIA Kit
CompanyCreative Diagnostics
Price Supplier Page View Company Product Page
Catalog NumberDEIABL312
Quantity96T
Sample Typetissue cell cultures, serum
SensitivityThe analytical sensitivity (LLOD) of this MMAE ADC EIA as determined by the 2 times standard deviation below the mean of B0 on 8 duplicate determinations of zero standard (B0) is approximately 0.0435 µg/mL
ReactivityHuman, Rat, Mouse, Primate
ComponentsThis test kit must be stored at 2 - 8°C upon receipt. For the expiration date of the kit refer to the label on the kit box. All components are stable until this expiration date.
Prior to use allow all reagents to come to room temperature.
Regents from different kit lot numbers should not be combined or interchanged.
1. Anti-MMAE Antibody Coated Microplate
One microplate with twelve by eight strips (96 wells total) coated with specific anti-MMAE antibody. The plate is framed and sealed in a foil zipper bag with a desiccant. This reagent should be stored at 2 - 8°C and is stable until the expiration date on the kit box.
2. HRP Conjugated MMAE
One vial containing 3 mL of ready to use HRP labeled MMAE in a stabilized protein matrix. This reagent should be stored at 2 - 8°C and is stable until the expiration date on the kit box.
3. ELISA Wash Concentrate
One bottle containing 30 mL of 30-fold concentrate. Before use the contents must be diluted with 870 mL of demineralized water and mixed well. Upon dilution, this yields a working wash solution containing a surfactant in phosphate buffered saline with a non-azide, non-mercury preservative. The diluted wash solution may be stored at room temperature and is stable until the expiration date on the kit box.
4. ELISA HRP Substrate
One bottle containing 15 mL of tetramethylbenzidine (TMB) with hydrogen peroxide. This reagent should be stored at 2 - 8°C and is stable until the expiration date on the kit box.
5. ELISA Stop Solution
One bottle containing 15 mL of stop solution. This reagent may be stored at 2 - 8°C or room temperature and is stable until the expiration date on the kit box.
6. Antibody-MMAE Conjugated Zero Calibrator (Cat. No. CD41822)
One vial containing 30mL zero calibrator (CD41822). This reagent is used for diluting the calibration stock t make assay calibrators, as well as for diluting test samples. This reagent should be stored at 2-8°C and is stable until the expiration date on the kit box.
7. Antibody-MMAE Conjugated Calibration Stock (Cat. No. CD41823) - Not provided in the kit (optional)
One vial (CD41823) containing the calibration stock of antibodyMMAE-conjugate in a lyophilized (0.5 mL) serum based matrix with a non-azide preservative. Refer to the vial for exact concentration of the standard. This reagent should be stored at 2 - 8°C and is stable until the expiration date on the kit box
PrecisionThe intra-assay precision was validated by measuring three calibrators (L3, L6 and L8) in eight replicate determinations. The CV% is 7.1%, 6.7% and 11.0%
ApplicationsQuantitative